Ablett, 26, has played 133 games over eight seasons at the Swans, including the 2005 premiership.
A chronic hamstring complaint has restricted Playfair, 26, to just 11 games in his two seasons at the club since crossing from Geelong in 2007.
He failed to play any football in 2009 while battling to recover from hamstring surgery.
Despite their delisting, Swans coach Paul Roos said both Ablett and Playfair could yet be at the club in 2010, depending on its strategy for the national, pre-season and rookie drafts.
"We've delisted them to give them a chance elsewhere but both of them are still training here," he said on Friday.
"We'll assess them against how many picks we want, and what Luke or Henry brings compared to a young player coming into the list.
"They're still training and very much in the mix to be part of the team."
